Purpose:
To provide the AIRR Community with biological calibrators and reagents for evaluation of AIRR-seq.
The Biological Resources Working Group is responsible for coordinating the development of reference samples that can be used as controls. The working group aims at reaching out to established organizations such as NIST and Genome in a Bottle, as well as companies to help encourage ease of use and broad adoption.
Goals:
The goal of the working group is to be able to provide AIRR biological standards and protocol recommendations to the scientific and biomedical community. Recommendation of a set of biological standards that can be used for normalization of data sets will allow more direct comparison of data generated by different library prep methods.
Plans for 2022/23 include:
- Generation of data from different BCR sequencing methods using three different types of standards: synthetic RNA templates, spleen DNA/RNA and cell line mixtures
- Generation of data from TCR sequencing standards comparison, starting from the methods that performed best in the Nature Biotechnology publication that was led by several of the WG members (Barennes et al., 2021)
- Continued participation in FDA BCR-SEQC initiative
- Continued participation in EuroClonality WG and meetings
- Generation of data and publication of a paper about sequencing depth and sample optimization for unique molecular identifiers (UMIs)
Long term:
- Evaluate products
- Outreach to academic and commercial partners
- Produce and share selected calibrators and reagents
- Labs/protocols for production
- Legal agreements for distribution
- Lab survey on standard B cell and T cell samples, including calibrators (MiAIRR data compliant)
- Define statistical measures (in collaboration with other WGs)
Products:
- Validated reagents
- Publication: review on existing reagents and application
- Publication in eLife (Trück et al., 2021)
Members:
Co-leaders: Anne Eugster, Nina Luning Prak and Johannes Trück
Members: Houda Alachkar, Davide Bagnara, John Beckford, Anne Eugster (Co-lead), Nina Luning Prak (Co-lead), Encarnita Mariotti-Ferrandiz, Johannes Trück (Co-lead), Nidhi Gupta, Andrew Farmer, Shaveta Goyal, Theam Soon Lim, Wenming Xiao.
