To provide the AIRR Community with biological calibrators and reagents for evaluation of AIRR-seq.
The Biological Resources Working Group is responsible for coordinating the development of reference samples that can be used as controls. The working group will reach out to established organizations such as NIST and Genome in a Bottle, as well as companies like ATCC and Novartis to help encourage ease of use and broad adoption.
The goal of the working group is to be able to recommend a set of biological standards that can be used for normalization of data sets. This will allow more direct comparison of data generated by different library prep methods. Ultimately they would like to have DNA and RNA spike-ins, in addition to cell mixes that can be used for this purpose.
Plans for 2021 include:
- Apply for funding
- Initiate a comparative study for B-cell AIRR-seq methods
- to work out existing differences between approaches
- to define what calibrators need to be developed
- Identify and collect suitable DNA/RNA calibrators/controls and cell rises for AIRR-seq
- identify members of the community able to define statistical measurement for AIRR-seq experiment validation (in collaboration with other WGs)
- perform inter-lab comparison
- perform outreach to academic, government, and commercial partners to disseminate the identified and validated controls
- define legal requirements for distribution
- Evaluate products
- Outreach to academic and commercial partners
- Produce and share selected calibrators and reagents
- Labs/protocols for production
- Legal agreements for distribution
- Lab survey on standard B cell and T cell samples, including calibrators (MiAIRR data compliant)
- Define statistical measures (in collaboration with other WGs)
- Validated reagents
- Publication: review on existing reagents and application
Co-leaders: Anne Eugster, Cinque Soto and Johannes Trueck
Members: Francisco Arcila, Davide Bagnara, Pierre Bareness, Maggie Bostick, Christian Busse, Elaine Chen, Lindsay Cowell, Anne Eugster (Co-lead), Marie Paule Lefranc, Nina Luning Prak, Encarnita Mariotti-Ferrandiz, Cinque Soto (Co-lead), Chris Tipton, Johannes Trüeck (Co-lead), and Ian York.