The Inferred Allele Review Sub-committee (IARC) was formed after the third AIRR-Community meeting in Rockville, MD in December 2017. The IARC is responsible for judging the validity of germline immunoglobulin and TCR genes, inferred from RepSeq data. It advises IMGT and the IUIS/IMGT nomenclature committee of their findings. It also reviews inferred sequences, and evidence in support of their existence, and makes this data available to the AIRR community and other researchers through the OGRDB database. The work of the committee initially focused on human IGHV germline genes, but it is hoped that work will commence on the evaluation of other germline genes, and species.
- Begun collating data for inferred human IGHV alleles from interested researchers, and established a formal submission process.
- Developed criteria for the review and acceptance of inferred IGHV alleles.
- With William Lees and Adrian Shepherd of Birkbeck, University of London, co-developed OGRDB, a public resource for the submission, review and publication of previously unknown receptor germline sequences together with supporting evidence.
- In its first year of operation, 16 new IGHV alleles were approved by the IARC. All 16 sequences were subsequently given names by IUIS/IMGT Nomenclature Committee, and have been incorporated into the IMGT Reference Directory.
Further details, including evidence in support of the inferences, can be found at the OGRDB website.
Goals for 2021
- Continue to evaluate human IGH IGK, and IGL inferences
- Establish a human TCR IARC
- Establish IARC(s) for non-human species
- Develop tools and guidelines for the evaluation of the ‘quality’ of submitted datasets
- Streamline procedures for the submission and assessment of human IGHV inferences
- Develop strategies to make the work of the IARC(s) sustainable
Co-leader: Andrew Collins
Members: Andrew Collins (Co-lead), Martin Corcoran, William Lees, Mats Ohlin, Corey Watson and Gur Yaari.
The OGRDB database documents the evaluation process of each submitted gene sequence, and provides access to evidence in support of affirmed inferences.
The VDJbase database of genotypes and haplotypes is being developed in association with OGRDB, and provides evidence of the expression of germline genes in hundreds of individuals.
Discussion of some issues arising from activities of the Germline Database Working Group can be found at the B-T.CR Wiki.